R&D Systems Recombinant Human TGF-beta 1 Protein CF
R&D Systems Recombinant Human TGF-beta 1 Protein CF is a new TGF-beta 1 that is Hek293 expressed.
Manufacturer: R&D Systems 240B500/CF
DescriptionMeasured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED 50 for this effect is 0.04-0.2 ng/mL. The specific activity of Recombinant Human TGF-beta 1 is approximately 2.5 x 10 4 U/µg, which is calibrated against human TGF-beta 1 Standard (NIBSC code: 89/514).
|>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.|
|Chinese Hamster Ovary cell line, CHO-derived Ala279-Ser390|
|<0.10EU per 1μg of the protein by the LAL method.|
|Reconstitute 2μg vials at 20μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin. Reconstitute 10μg or larger vials at 100μg/mL in sterile 4 mM HCl.|
|Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA.|
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.|
|CEDLAP, DPD1, latency-associated peptide, TGFB, TGFB1, TGFbeta, TGFbeta 1, TGF-beta 1 protein, TGF-beta-1, transforming growth factor beta-1, transforming growth factor, beta 1|
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