DescriptionMeasured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED50 for this effect is 5-15 ng/mL.
|M.W. (Observed): 80-90 kDa, reducing conditions; M.W. (theoretical): 35 kDa|
|>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.|
|Mouse myeloma cell line, NS0-derived human Thrombopoietin/Tpo protein Ser22-Gly353|
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.|
|Lyophilized from a 0.2 µm filtered solution in PBS.|
|<0.1 EU per 1 µg of the protein by the LAL method.|
|Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, megakaryocyte stimulating factor, MGDF, MGDFC-mpl ligand, MKCSF, MK-CSF, ML, MPL ligand, MPLLG, MPLLGMGC163194, Myeloproliferative leukemia virus oncogene ligand, THCYT1, THPO, thrombopoietin, thrombopoietin nirs variant 1, Tpo, TPOMKCSF|
|Reconstitute at 100 µg/mL in sterile PBS.|
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