R&D Systems™ Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF
Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. ED50 for this effect is 20 to 100 pg/mL.
Manufacturer: R&D Systems™ 10291TA01M
|APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha|
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.|
|Reconstitute at 500 µg/mL in PBS.|
|<0.10 EU per 1 µg of the protein by the LAL method.|
|>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.|
|Human embryonic kidney cell, HEK293-derived human TNF-alpha protein Val77-Leu233|
|Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose.|
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