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R&D Systems™ Recombinant Human TNF-alpha Hyperactive Mutant Protein, CF

Mutant form with approximately 5-fold greater activity

Specifications

Accession Number P01375
Buffer Lyophilized from a 0.2 µm filtered solution in PBS.
Conjugate Unconjugated
Endotoxin Level <0.10 EU per 1 µg of the protein by the LAL method.
For Use With (Application) Bioactivity
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
85-99T-A025CF
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R&D Systems™
8599TA025/CF
25 µg This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000. N/A
85-99T-A100CF
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R&D Systems™
8599TA100/CF
100 µg This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000. N/A
Description

Description

  • Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.
  • ED50 for this effect is 20 to 100 pg/mL.
Specifications

Specifications

P01375
Unconjugated
Bioactivity
17 kDa
>95%, by SDS-PAGE with silver staining.
E. coli-derived human TNF-alpha protein Lys87-Leu233 (Leu233Phe), with an N-terminal Met-Arg-Lys-Arg
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 µg of the protein by the LAL method.
7124
APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
Reconstitute at 100 µg/mL in PBS.
Human
SDS
Product Certifications
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