R&D Systems™ Recombinant Mouse Pentraxin 2/SAP His-tag Protein, CF

Measured by its binding ability in a functional ELISA.

Supplier:  R&D Systems™ 2558SAB050

Catalog No. 25-58S-AB050

Description

When recombinant Mouse Pentraxin/SAP His-tag (2558-SAB) is immobilized at 10 μg/mL (100 μL/well), the concentration of Biotinylated recombinant human FC gamma RIIA/CD32a (R167) Protein that produces 50% of the optimal binding response is found to be approximately 0.5-2.5 μg/mL.

Specifications

• Accession Number: P12246
• Gene ID (Entrez): 325
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• Source: Mouse myeloma cell line, NS0-derived mouse Pentraxin 2/SAP protein Gln21-Asp224, with a C-terminal 10-His tag
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Reconstitution: Reconstitute at 100 μg/mL in sterile water.

• Formulation: Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and CaCl2.
• Molecular Weight (g/mol): Observed: 27-35 kDa, under reducing conditions; Theroretical: 25 kDa
• Quantity: 50 μg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Conjugate: Unconjugated
• Protein: Pentraxin 2/SAP