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R&D Systems™ Recombinant Mouse Pentraxin 2/SAP His-tag Protein, CF
Measured by its binding ability in a functional ELISA.
Supplier: R&D Systems™ 2558SAB050
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DescriptionWhen recombinant Mouse Pentraxin/SAP His-tag (2558-SAB) is immobilized at 10 μg/mL (100 μL/well), the concentration of Biotinylated recombinant human FC gamma RIIA/CD32a (R167) Protein that produces 50% of the optimal binding response is found to be approximately 0.5-2.5 μg/mL.
|>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.|
|Mouse myeloma cell line, NS0-derived mouse Pentraxin 2/SAP protein Gln21-Asp224, with a C-terminal 10-His tag|
|<0.10 EU / 1 μg of the protein by the LAL method.|
|Reconstitute at 100 μg/mL in sterile water.|
|Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and CaCl2.|
|Observed: 27-35 kDa, under reducing conditions; Theroretical: 25 kDa|
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.|
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