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Thermo Scientific™ Simmons Citrate Agar
Differentiate gram-negative bacteria based on citrate utilization using Simmons Citrate Agar.
Supplier: Thermo Scientific™ R060496
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Description
Differentiate gram-negative bacteria based on citrate utilization using Thermo Scientific™ Remel™ Simmons Citrate Agar. In 1923, Koser developed a liquid medium to differentiate Escherichia coli and Enterobacter aerogenes1. The medium contained an inorganic ammonium salt and sodium citrate that provided a single nitrogen source and a single carbon source respectively. In 1926, this formulation was modified by Simmons who added 1.5% agar and brom thymol blue indicator2.
Gram-negative bacteria that can utilize ammonium dihydrogen phosphate as a sole nitrogen source and sodium citrate as a sole carbon source will grow on Citrate Agar (Simmons). These organisms extract nitrogen from the ammonium salt by breaking it down
Specifications
| Industrial Reference: AOAC, BAM, COMPF, USDA. | |
| 15mm x 103mm Tube - Slant | |
| 20 x 5 mL |
| Simmons Citrate Agar | |
| Agar |
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