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Thermo Scientific™ Sulfuric Acid 5% Decolorizer

Use Sulfuric Acid 5% Decolorizer as a reagent in acid-fast staining procedures to detect Cryptosporidium in clinical specimens.

Supplier:  Thermo Scientific™ R40125

Catalog No. R40125


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Description

Description

Use Thermo Scientific™ Remel™ Sulfuric Acid 1% Decolorizer in the acid-fast staining procedure to detect Cryptosporidium in clinical specimens.

Cryptosporidium is an intestinal protozoan parasite that causes diarrhea in animals as well as humans. This strain causes infection in immunocompromised hosts, especially those with AIDS (Acquired Immune Deficiency Syndrome)1. A modification of the Ziehl-Neelsen stain that utilizes sulfuric acid 5% is recommended by Garcia to detect Cryptosporidium oocysts in clinical specimens2.

Cryptosporidium oocysts are spherical 4-6 μm in diameter, and stain pink to red to purple with Ziehl-Neelsen carbolfuchsin stain. Most will contain several dark staining granules. Yeast cells which are most likely to be confused with Cryptosporidium oocysts, stain blue with the counterstain, TB Methylene Blue. Sulfuric Acid 5% decolorizes the primary stain.

Cryptosporidium is more difficult to detect in formed stool specimens. Stain time is increased that allows the oocysts to stand out from the background material. The hot acid-fast stain maximizes detection and identification of Cryptosporidium in formed stools2.

  • Easy-to-use- Examine smear in the slides mounted with mounting medium and a coverslip by bright field microscopy (40 X objective) and oil immersion is used (100 X objective) to observe the internal morphology.
  • Easy-to-interpret- In case of positive test oocysts stain pink to red to purple, are 4-6 μm in diameter and round or oval in shape. One to four sporozoites maybe visible within the oocyst. In case of negative test the yeast cells and background material stain blue.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

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Specifications

Specifications

Decolorizer
250 mL/Bottle
Each
SDS
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