Thermo Scientific™ Tetrathionate Broth

Catalog Number	Mfr. No.	Quantity
R07162	Thermo Scientific™ R07162	20 x 10 mL
R07160	Thermo Scientific™ R07160	100 x 10 mL

Description

Selectively isolate Salmonella from fecal specimens using Thermo Scientific™ Remel™ Tetrathionate Broth enrichment medium. This broth was originally described by Muller for cultivation of Salmonella spp¹. He demonstrated coliforms were inhibited in the medium while certain enteric gram-negative bacilli, such as Salmonella spp. grew very well. Kauffmann reported an increase in the recovery rate of Salmonella in the Muller medium^2,3.

Salmonella are a group of closely related rod-shaped, gram-negative bacteria. Salmonella typically live in animal and human intestines and are shed through feces. Humans become infected most frequently through contaminated water or food. The most common symptoms of Salmonella infection are diarrhea, fever, abdominal cramps, and vomiting.

Tetrathionate broth, when supplemented with Iodine for Tetrathionate, is a selective enrichment medium that is recommended for isolation of Salmonella from fecal specimens.

• Ready-to-use – The convenience of prepared media.
• Selective – Bile salts and sodium thiosulfate are selective agents which inhibit gram-positive organisms and some enteric gram-negative bacilli.
• Formulated as per specifications of the American Public Health Association (APHA), the Association of Official Analytical Chemists (AOAC), the United States Department of Agriculture (USDA), and the United States Pharmacopeia (USP).^4-7

This medium contains casein and meat peptones that provide nutritious sources of nitrogen, amino acids, and peptides necessary for bacterial growth. Tetrathionate is formed by the addition of a solution containing iodine and iodide. Organisms capable of reducing tetrathionate, such as Salmonella, flourish in the medium while other fecal organisms are inhibited. Bile salts and sodium thiosulfate are selective agents which inhibit gram-positive organisms and some enteric gram-negative bacilli.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

Specifications

• Certifications/Compliance: Industrial Reference: AOAC, BAM, COMPF, SMWW, USDA, USP.
• Description: Tetrathionate Broth
• Product Type: Microbiology Media

Requires addition of Iodine Solution, REF R21228, REF R114350, or REF R114351. REF R112634 and REF R112630 not intended for IVD use.