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Thermo Scientific™ RevertAid™ Reverse Transcriptase Promotion

Perform efficient synthesis of full-length first strand cDNA up to 13 kb with this genetically modified M-MuLV RT for routine reverse transcription.

Manufacturer:  Thermo Scientific™ EP0441

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Catalog No. FEREP0441PM

This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
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Description

Description

Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Highlights

  • Efficient synthesis of full-length first strand cDNA up to 13 kb
  • Optimum activity at 42°C
  • Active up to 50°C
  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)
Specifications

Specifications

First strand cDNA synthesis for RT-PCR and real-time RT-PCR; Synthesis of cDNA for cloning and expression; Generation of labeled cDNA probes for microarrays; DNA labeling (3)
10 x 1mL of 5X Reaction Buffer
RevertAid Reverse Transcriptase
10,000U
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