Thermo Scientific RevertAid Reverse Transcriptase Promotion
Perform efficient synthesis of full-length first strand cDNA up to 13 kb with this genetically modified M-MuLV RT for routine reverse transcription.
Manufacturer: Thermo Scientific EP0441
Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.
- Efficient synthesis of full-length first strand cDNA up to 13 kb
- Optimum activity at 42°C
- Active up to 50°C
- Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)
|First strand cDNA synthesis for RT-PCR and real-time RT-PCR; Synthesis of cDNA for cloning and expression; Generation of labeled cDNA probes for microarrays; DNA labeling (3)|
|10 x 1mL of 5X Reaction Buffer|
|RevertAid Reverse Transcriptase|
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