RNA Polymerase II/POLR2A Mouse anti-Human, Mouse, Yeast, Primate (Negative), PE/Cy5.5, Clone: 4H8, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer:  Novus Biologicals NB200598PECY55

Catalog No. N20598PECY5

Description

RNA Polymerase II/POLR2A Monoclonal antibody specifically detects RNA Polymerase II/POLR2A in Human, Mouse, Yeast, Primate (Negative) samples. It is validated for Flow Cytometry.

Specifications

• Antigen: RNA Polymerase II/POLR2A
• Clone: 4H8
• Dilution: Flow Cytometry
• Format: Conjugated, Purified
• Gene Symbols: POLR2A
• Immunogen: 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). [UniProt No. P24928]
• Purification Method: Protein G purified
• Research Discipline: Chromatin Research, Epigenetics, microRNA, Phospho Specific, Transcription Factors and Regulators
• Primary or Secondary: Primary
• Gene ID (Entrez): 5430.0
• Target Species: Human, Mouse, Primate (Negative), Yeast

• Applications: Flow Cytometry
• Conjugate: PE-Cyanine5.5
• Formulation: PBS with 0.05% Sodium Azide
• Gene Alias: DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, EC 2.7.7.48, EC 2.7.7.6, hRPB220, hsRPB1, MGC75453, POLR2, POLRA, polymerase (RNA) II (DNA directed) polypeptide A (220kD), polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, RNA Pol 2, RNA Polymerase 2, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RNAPII, RPB1, RPBh1, RpIILS, RPO2, RPOL2
• Host Species: Mouse
• Isotype: IgG1
• Quantity: 0.1 mL
• Storage Requirements: Store at 4°C in the dark. Do not freeze.
• Monoclonal or Polyclonal: Monoclonal
• Test Specificity: Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein. When using this antibody in western blot and IP, it recognizes both the II0 and IIA form of RPB1 (ie the largest RNAPII subunit). These different forms of RPB1 (as seen in gels) have traditionally been loosely defined as ′hyper-′ and ′hypo-phosphorylated′, respectively. Here, however, it is important to remember that RPB1 contains no less than 52 repeats of a heptapeptide (TSPTSPS) that can be phosphorylated at different positions (Tyr1, Ser2, Thr4, Ser5 and Ser7; this antibody was raised against a synthetic peptide with 10 of these repeats, mostly but probably not entirely, chemically phosphorylated at Ser5). So, when we say that it recognizes both forms (II0/hyper-phos and IIA/hypo-phos), it might really just mean that the antibody recognizes a few phosphorylated Ser5s among 52 otherwise mostly un-phosphorylated repeats. Alternatively, it might conceivably (though less likely) recognize UN-phosphorylated repeats as there might conceivably be a few of those even in the II0/hyper-phos RPB1 form.′