Invitrogen™ RNAqueous™ Total RNA Isolation Kit  

Uses glass fiber filter separation technology

Manufacturer:  Invitrogen™ AM1912

Catalog No. AM1912

Description

The RNAqueous Kit is for phenol-free total RNA isolation from 0.1g to 0.5g of tissue or from 10⁷ to 10⁸ cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.

• Convenient syringe-based filtration
• Compatibility with vacuum manifolds when processing multiple samples

How it works
The RNAqueous Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.

Not high-throughput compatible (Manual)

Applications:

DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues

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Specifications

• Final Product Type: Total RNA
• Isolation Technology: Spin Column (Glass Fiber Filter)
• Sample Type: Plant Samples, Viral Samples, Tissue, Yeast, Bacteria, Cells
• Product Type: Kit
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Product Line: Ambion™, RNAqueous™

• For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting, cDNA Library Construction
• Purification Target: Total RNA
• Includes: 6mL Formaldehyde Load Dye; 60mL Lysis/Binding Solution; 60mL Water for 64% Ethanol; 4mL Lithium Chloride Precipitation Solution; 40mL Wash Solution 1; 80mL Wash Solution 2/3 Concentrate; 50 Filter Cartridges; 100 Collection Tubes; 10mL Elution Solution
• Content And Storage: • 6 ml Formaldehyde Load Dye (-20°C)
  • 60 ml Lysis/Binding Solution (4°C)
  • 60 ml Water for 64% Ethanol (4°C)
  • 4 ml Lithium Chloride Precipitation Solution (4°C)
  • 40 ml Wash Solution 1 (4°C)
  • 80 ml Wash Solution 2/3 Concentrate (4°C)
  • 50 Filter Cartridges (room temperature)
  • 100 Collection Tubes (room temperature)
  • 10 ml Elution Solution (-20°C, 4°C, or room temperature)
• Shipping Condition: Room Temperature