MilliporeSigma RNF168, Rabbit, Unlabeled, Polyclonal,
Rabbit Polyclonal Antibody
Manufacturer: MilliporeSigma 061130I
DescriptionE3 ubiquitin-protein ligase RNF168 (UniProt Q8IYW5; also known as hRNF168, RING finger protein 168) is encoded by the RNF168 gene (Gene ID 165918) in human. Ubiquitylation/Ubiquitination is an essential early signal in the DNA damage repair process. Multiple E3 ligases, including RNF2 (RING2), RNF8 and RNF168, mediate the addition of K63-linked polyubiquitin chains to histone 2A (H2A) and H2AX, which in turn recruits DNA damage repair proteins, e.g. RAP80, BRCA1. In addition, SUMOylation and NEDDylation are also involved in the DNA damage response. RNF168 is an a dual specificity E3 ligase that mediates both the ubiquitination and NEDDylation of H2A and H2AX. The two types of modifications compete against each other and NEDD8 modification of H2A and H2AX blocks the recruitment of BRCA1 at DNA damage repair sites. RNF168 itself is also subjected to NEDD8 modification and NEDDylation of RNF168 is necessary for its ubiquitin ligase activity. Inhibition of RNF168 NEDDylation impairs its interaction with E2 enzyme Ubc13 (UBE2N). Likewise, downregulating RNF168 NEDDylation by the deNEDDylating enzyme NEDP1, or due to mutations of the NEDD8-conjugating enzyme UBC12, decreases H2A and H2AX ubiquitylation. RNF168 Mutations are linked to RIDDLE (radiosensitivity, immunodeficiency dysmorphic features and learning difficulties) syndrome.
|Please refer to lot specific datasheet.|
|Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.|
|Stable for 1 year at 2°-8°C from date of receipt.|
|This polyclonal antibody detected nuclear RNF168 immunoreactivity in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized human B-cell hybridoma SA13.|
|Immunoprecipitation, Western Blot|
|GST-tagged full-length human RNF168.|
|Epigenetics & Nuclear Function|
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For Research Use Only. Not for use in diagnostic procedures.