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ROR gamma (t) Rat anti-Human, Mouse, Rhesus Monkey, APC, Clone: AFKJS-9, eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

Manufacturer:  Invitrogen 17698882

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Catalog No. 50-112-8730


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Description

Description

Description: The AFKJS-9 monoclonal antibody reacts with the mouse, human and rhesus monkey RORgamma(t) protein. RORgamma is a member of the retinoic acid-related orphan receptor (ROR) family, which also includes RORalpha and RORbeta. ROR family proteins are ligand-dependent transcription factors that play roles in multiple physiological processes. RORgamma is expressed in several tissues including liver, lung, muscle, heart and kidney. Furthermore, it was discovered that alternative transcription results in the expression of an isoform, RORgammat, which is expressed exclusively in cells of the lymphoid compartment, namely CD4+CD8+ "double-positive" thymoyctes, Th17 cells of the periphery and lymphoid tissue inducer (Lti) cells of lymphoid organs. The RORgammat isoform differs from RORgamma by three unique amino acids at its amino terminus. Therefore, the AFKJS-9 antibody will react with both the RORgamma and RORgammat isoforms. Applications Reported: This AFKJS-9 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This AFKJS-9 antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online.

This antibody can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered. ROR gamma, a NR1 Thyroid Hormone-Like receptor, has been shown to affect thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. ROR gamma has been shown to promote thymocyte survival by activating the expression of the antiapoptotic protein Bcl-x(L). ROR gamma is also required for the development of lymph nodes and Peyer ft.s patches. It has been shown that ROR gamma t, a thymus-specific isoform of ROR gamma from mouse, inhibits Fas ligand expression and cytokine secretion in immature thymocytes. ROR gamma binds as a monomer to response elements composed of a single core motif, GGTCA, preceded by a 6 bp AT-rich sequence. ROR gamma expression has been documented in mouse thymus, adipose, bone, skeletal muscle, liver, and kidney, and in human skeletal muscle. ESTs have been isolated from human tissue libraries, including cancerous head/neck, germ cell, and prostate, and normal colon, head/neck, lung, liver/spleen, and pancreas.
Specifications

Specifications

ROR gamma (t)
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P51450, P51449
MGC129539; NR1F3; RORG; RZR-GAMMA; RZRG; TOR; RORgt
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Human, Mouse, Rhesus Monkey
Flow Cytometry
AFKJS-9
APC
RORC
Liquid
RORC
IgG2a
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
19885, 6097, 717052
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