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ROR gamma (t) Rat anti-Mouse, Clone: B2D, eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

Manufacturer:  Invitrogen 14698182

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Catalog No. 50-135-63


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Description

Description

Description: The B2D monoclonal antibody reacts with the mouse RORgamma(t) protein. RORgamma is a member of the retinoic acid-related orphan receptor (ROR) family, which also includes RORalpha and RORbeta. ROR family proteins are ligand-dependent transcription factors that play roles in multiple physiological processes. RORgamma is expressed in several tissues including liver, lung, muscle, heart and kidney. Furthermore, it was discovered that alternative transcription results in the expression of an isoform, RORgammat, which is expressed exclusively in cells of the lymphoid compartment, namely CD4+CD8+ "double-positive" thymoyctes, Th17 cells of the periphery and lymphoid tissue inducer (Lti) cells of lymphoid organs. The RORgammat isoform differs from RORgamma by three unique amino acids at the amino terminus. Therefore, the B2D antibody will react with both the RORgamma and RORgammat isoforms. Applications Reported: This B2D antibody has been reported for use in immunoblotting (WB). Applications Tested: This B2D antibody has been tested by western blot analysis of mouse thymus nuclear lysates. This B2D antibody can be used at a starting concentration of 2 µg/mL, however the optimal concentration should be determined for individual experimental conditions. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.

ROR gamma, a NR1 Thyroid Hormone-Like receptor, has been shown to affect thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. ROR gamma has been shown to promote thymocyte survival by activating the expression of the antiapoptotic protein Bcl-x(L). ROR gamma is also required for the development of lymph nodes and Peyer ft.s patches. It has been shown that ROR gamma t, a thymus-specific isoform of ROR gamma from mouse, inhibits Fas ligand expression and cytokine secretion in immature thymocytes. ROR gamma binds as a monomer to response elements composed of a single core motif, GGTCA, preceded by a 6 bp AT-rich sequence. ROR gamma expression has been documented in mouse thymus, adipose, bone, skeletal muscle, liver, and kidney, and in human skeletal muscle. ESTs have been isolated from human tissue libraries, including cancerous head/neck, germ cell, and prostate, and normal colon, head/neck, lung, liver/spleen, and pancreas.
Specifications

Specifications

ROR gamma (t)
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
P51450
MGC129539; NR1F3; RORG; RZR-GAMMA; RZRG; TOR; RORgt
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Western Blot
B2D
Unconjugated
Rorc
Liquid
Rorc
IgG1, kappa
100 μg
4° C
Primary
19885
SDS
Documents
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