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Thermo Scientific™ RPMI 1640 Media for SILAC

Analyze protein expression by mass spectrometry using these media or additives for stable isotope labeling with amino acids in cell culture (SILAC).

$58.55 - $235.56

Specifications

Product Type RPMI 1640 Media for SILAC
For Use With (Application) Protein mass spectrometry analysis
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Products 2
Catalog Number Mfr. No. Quantity Material Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Material Price Quantity & Availability  
PI88365 View Documents Thermo Scientific™
88365
500mL Protein samples Each for $58.55
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PIA33823 View Documents Thermo Scientific™
A33823
6 x 500mL Cell Lysate Each for $235.56
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Description

Description

Thermo Scientific RPMI Media for SILAC is optimized for use with stable isotope labeling with amino acids in cell culture (SILAC) to analyze protein expression by mass spectrometry (MS).

Stable isotope labeling with amino acids in cell culture (SILAC) is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of “heavy” 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

This SILAC media is used together with heavy and light amino acids for the differential isotopic labeling of cells for SILAC analysis. SILAC media supplemented with heavy amino acids are capable of nearly 100% label incorporation into proteins of living cells.

Features of RPMI Media for SILAC:
  •  Flexible—liquid media deficient in both L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling
  • High quality—media are sterile, endotoxin-free and cell culture-compatible
Specifications

Specifications

RPMI 1640 Media for SILAC
Protein mass spectrometry analysis
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