Invitrogen™ RTN1 Monoclonal Antibody (RNL-2)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA106813

Catalog No. MA106813

Description

MA1-06813 detects reticulon-1A(NSP-A) and reticulon-1B(NSP-B) in human, rabbit, and non-human primate cells. MA1-06813 has sucessfully been used in Western blotting, immunocytochemistry, FACS and immunohistochemistry (paraffin) procedures. By Western blot it detects a ~135 kDa band representing reticulon-1A, and a ~45 kDa band representing reticulon-1B. The MA1-06813 immunogen is small cell lung cancer cell line NCI-H82.

Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. reticulon-1A (NSP-A), reticulon-1B (NSP-B), and reticulon-1C (NSP-C). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight of approximately 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is so far not found in human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).

Specifications

• Antigen: RTN1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.09% sodium azide
• Gene Accession No.: Q16799
• Gene Symbols: RTN1
• Immunogen: Small cell lung cancer cell line NCI-H82.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Rabbit, Monkey
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: RNL-2
• Conjugate: Unconjugated
• Gene: RTN1
• Gene Alias: 0710005K15Rik; 4930441F12Rik; I79_024631; LOC100750648; MGC133250; MGC40985; Neuroendocrine-specific protein; Nsp; R75279; reticulon 1; reticulon-1; RP23-450G14.1; RTN1; Rtn1-a; Rtn1-b; Rtn1-c; S-rex
• Host Species: Mouse
• Purification Method: Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 100345452, 6252
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov