S100A8 Monoclonal Antibody (CF-145), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen 14974582

Catalog No. 50-112-2814

Description

Description: The monoclonal antibody CF-145 recognizes human S100A8. S100A8, also known as migration inhibitory factor-related protein 8 (MRP-8) or Calgranulin-A, is a calcium binding protein and member of the S100 family of proteins. S100A8 is expressed by neutrophils, monocytes, macrophages, granulocytes, and epithelial cells during dermatoses, such as psoriasis. S100A8 forms a heterodimer with S100A9, which together is known as calprotectin. S100A8 has antimicrobial and pro-apoptotic properties and is a pro-inflammatory mediator in acute and chronic inflammation. Levels of S100A8 are elevated in patients with various inflammatory disorders such as cystic fibrosis, rheumatoid arthritis, and chronic bronchitis. Upregulation of S100A8 is also observed in many tumors, including skin, breast, colon, pancreatic, and ovarian cancers. Applications Reported: This CF-145 antibody has been reported for use in western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This CF-145 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low pH antigen retrieval and can be used at less than or equal to 1 μg/mL. This CF-145 antibody has been tested by western blot of A375 cell lysate and can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.

Specifications

• Antigen: S100A8
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P05109
• Gene Symbols: S100A8
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 6279
• Content And Storage: 4° C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Clone: CF-145
• Conjugate: Unconjugated
• Gene: S100A8
• Gene Alias: 60B8AG; AI323541; B8Ag; CAGA; CAGB; calgranulin A; calgranulin B; calgranulin-A; Calgranulin-B; calprotectin L1H subunit; Calprotectin L1L subunit; CFAg; CGLA; CGLB; chemotactic cytokine CP-10; chemotactic S100 protein; CP-10; Cystic fibrosis antigen; L1Ag; leukocyte L1 complex heavy chain; Leukocyte L1 complex light chain; LIAG; MA387; MAC387; MIF; Migration inhibitory factor-related protein 14; migration inhibitory factor-related protein 8; MRP14; MRP-14; MRP8; MRP-8; NIF; P14; p8; Pro-inflammatory S100 cytokine; protein S100-A8; Protein S100-A9; S100 A8; S100 calcium binding protein A8; S100 calcium binding protein A8 (calgranulin A); S100 calcium binding protein A9; S100 calcium-binding protein A8; S100 calcium-binding protein A8 (calgranulin A); S100 calcium-binding protein A9; S100 calcium-binding protein A9 (calgranulin B); S100A8; S100A9; Urinary stone protein band A
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2b κ

For Research Use Only.