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Human TNF alpha Animal Free, Recombinant Protein, eBioscience™ Non-distribution product as customer accommodation.

Recombinant Protein

Manufacturer:  Invitrogen 68878682

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Catalog No. 50-112-4915


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Description

Description

Tumor Necrosis Factor-Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor-bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single ~17kDa band following SDS-PAGE under non-reducing conditions. Description: This SAFE™ (Serum and Animal Free, low Endotoxin) protein has been made in plants with no exposure to animal protein during expression or purification. This animal free product has very low levels of endotoxin. Human TNF alpha is a memeber of the TNF ligand superfamily produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 24kDa form of TNF alpha is expressed as a membrane bound molecule. TNF alpha is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF alpha have been observed. Human and mouse TNF alpha conservation is about 79%, Human protein is functional on mouse, although with slightly reduced activity. Applications Reported: SAFE™ Human TNF alpha Recombinant Animal Free Protein is biologically active. Applications Tested: The ED50 of this protein, as measured by cytolystic activity of actinomycin-D sensitized L929 cells, is less than or equal to 0.4ng/mL. This corresponds to a specific activity of greater than or equal to 2.5 x 10e6 Units/mg. Source: Plant expressed 158 amino acids (Val77-Leu233) with 16 amino acid Histidine tag. accession P01375. Bioactivity: The ED50 of this protein, as measured by cytolystic activity of actinomycin-D sensitized L929 cells, is less than or equal to 0.4ng/mL. This corresponds to a specific activity of greater than or equal to 2.5 x 10e6 Units/mg. Endotoxin: Less than 0.005ng/ug (0.05 EU/ug) as measured by turbidity assay. Purity: Greater than or equal to 95% as determined by SDS-PAGE. Molecular Weight: 24 kDa. Storage: The lyophilized powder can be stored at less than or equal to -20°C. (Short term storage at 2-8°C). Reconstitute in sterile PBS to a concentration no less than 100 ug/mL. Once reconstituted, protein should be aliquoted and stored at -20°C or lower. For best stability add 0.1% BSA before freezing aliquots.
Specifications

Specifications

P01375
Lyophilized
7124
Human TNF alpha Animal Free
SDS-PAGE
100μg
4°C or -20°C if preferred
Plant expressed 158 amino acids (Val77-Leu233) with 16 amino acid Histidine tag. Accession P01375
Less than 0.005ng/μg (0.05 EU/μg) as measured by turbitidity assay
TNF
The ED50 of this protein, as measured by cytolystic activity of actinomycin-D sensitized L929 cells, is less than or equal to 0.4ng/mL. This corresponds to a specific activity of greater than or equal to 2.5 x 106 Units/mg.
Human
Hu
Recombinant
Functional Assays
PBS with no preservative; pH 7.2
24kDa
7.2
Affinity chromatography
Plant
RUO
Recombinant
Human TNF alpha Animal Free
Control, Functional Assay
Recombinant Protein
Unlabeled
Plant
Safety and Handling

Safety and Handling

ShelfLife : 12 Months

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