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Invitrogen™ Sclerostin (SOST) Human ELISA Kit

Sandwich ELISA Kit

Manufacturer:  Invitrogen™ EHSOST

Encompass

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Catalog No. EHSOST


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Description

Description

The Human Sclerostin (SOST) ELISA quantitates Hu Sclerostin human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Sclerostin. Principle of the method The Human Sclerostin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.
Specifications

Specifications

ELISA Kit
96 assays
Solid-phase sandwich ELISA
Other Proteins
Q9BQB4
15pg/mL to 10000pg/mL
50964
RUO
2°C to 8°C
4 hr. 45 min.
Bone Research
<12%
96 Tests
Plasma, Serum, Supernatant
CDD,DAND61,VBCH,SOST
This ELISA antibody pair detects human SOST. Other species not determined yet.
Colorimetric Microplate Reader
Biotin
HRP
40.96-10,000 pg/mL
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
1 hr. 20 min.
Human
UNQ2976/PRO7455/PRO7476, CDD1, VBCH, SOST
<10%
Human
15 pg/mL
SDS
Documents
Product Certifications
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