Invitrogen™ SHC Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA579996

Catalog No. PIPA579996

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: human A431 whole cell, human Hela whole cell, human HepG2 whole cell, human Jurkat whole cell, human K562 whole cell, human THP-1 whole cell, rat C6 whole cell, mouse thymus tissue, mouse RAW2467 whole cell, mouse NIH3T3 whole cell. IHC: Mouse Brain tissue, Rat Brain tissue, Human Lung Cancer tissue IHC-F: Human Placenta tissue.

Skp2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex. Skp2 is essential for the G1-S transition in both transformed cells and diploid fibroblasts. Biochemical and genetic experiments have demonstrated that Skp2 is required for the ubiquitination and consequent degradation of p27 in cultured mammalian cells and in vitro reconstitution assays. In normal tissues, Skp2 is expressed in tonsil and placenta. In tumor tissues, Skp2 is expressed widely, in some colon, prostate, pancreas, and skin cancers, with high expression in lung, breast, ovarian, and endometrial cancers. Research suggests that Skp2 plays a role in oncogenesis, particularly in the pathogenesis of lymphomas and breast cancers.

Specifications

• Antigen: SHC
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SHC1
• Gene Alias: FLJ26504; OTTHUMP00000035472; p66; P66shc; RP11-307C12.1; SH2 domain protein C1; SHC; SHC (Src homology 2 domain containing) transforming protein 1; SHC (Src homology 2 domain-containing) transforming protein 1; SHC adaptor protein 1; SHC1; SHCA; SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A; src homology 2 domain-containing transforming protein C1; src homology 2 domain-containing-transforming protein C1
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 20416, 6464, 85385
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
• Concentration: 500 μg/mL
• Formulation: PBS with 5mg BSA and 0.05mg sodium azide
• Gene Accession No.: P29353, P98083, Q5M824
• Gene Symbols: SHC1
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human SHC (536-564aa VDPEGVVRTKDHRFESVSHLISYHMDNHL).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov