SHH Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA588595

Catalog No. PIPA588595

Description

Positive Samples: A-549; Cellular Location: Cell membrane, Lipid-anchor, extracellular space Immunogen sequence: GRLLYSDFLT FLDRDDGAKK VFYVIETREP RERLLLTAAH LLFVAPHNDS ATGEPEASSG SGPPSGGALG PRALFASRVR PGQRVYVVAE RDGDRRLLPA

Sonic Hedgehog (SHH), which is expressed only during embryogenesis, is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the Sonic Hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. In addition, it is thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.

Specifications

• Antigen: SHH
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Shh
• Gene Alias: 9530036O11Rik, Dsh, fc83d08, hedgehog, hemimelic extra toes, Hhg1, HHG-1, HLP3, HPE3, Hx, Hxl3, M100081, MCOPCB5, morphogen, shh, Shh N-terminal processed signaling domains, Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains, shh.L, shha, ShhN, ShhNC, ShhNp, short digits, SMMCI, sonic hedgehog, sonic hedgehog a, sonic hedgehog homolog, sonic hedgehog L homeolog, sonic hedgehog protein, Sonic hedgehog protein 19 kDa product, Sonic hedgehog protein A, Sonic hedgehog protein A N-product, Sonic hedgehog protein N-product, sonic hedgehog signaling molecule, sonic hedgehog signaling molecule a, sonic-you, syu, TPT, TPTPS, vhh1, vhh-1, wu:fc83d08, XELAEV_18030676mg, Xhh, xshh, X-SHH
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 6469
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Immunocytochemistry, Western Blot
• Concentration: 0.41 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide, pH 7.3
• Gene Accession No.: Q15465
• Gene Symbols: Shh
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 231-330 of human Sonic Hedgehog (Shh) (NP_0001841)
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov