SIGLEC8 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA587961

Catalog No. PIPA587961

Description

Immunogen sequence: MEGDRQYGDG YLLQVQELVT VQEGLCVHVP CSFSYPQDGW TDSDPVHGYW FRAGDRPYQD APVATNNPDR EVQAETQGRF QLLGDIWSND CSLSIRDARK RDKGSYFFRL ERGSMKWSYK SQLNYKTKQL SVFVTALTHR PDILILGTLE SGHSRNLTCS VPWACKQGTP PMISWIGASV SSPGPTTARS SVLTLTPKPQ DHGTSLTCQV TLPGTGVTTT STVRLDVSYP PWNLTMTVFQ GDATASTALG NGSSLSVLEG QSLRLVCAVN SNPPARLSWT RGSLTLCPSR SSNPGLLELP RVHVRDEGEF TCRAQNAQGS QHISLSLSLQ NEGTGTSRPV SQVTLAA; Positive Samples: HL-60, 293T, Mouse liver, Rat blood; Cellular Location: Membrane, Single-pass type I membrane protein

SIGLEC8 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2,3-linked sialic acid. and also binds to alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. SIGLEC8 is expressed specifically on eosinophils. The protein contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in downmodulation of cellular responses. The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1. The SIGLEC8 gene belongs to the immunoglobulin superfamily.

Specifications

• Antigen: SIGLEC8
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SIGLEC8
• Gene Alias: CDw329, MGC59785, SAF2, SAF-2, sialic acid binding Ig like lectin 8, sialic acid binding Ig-like lectin 8, sialic acid-binding Ig-like lectin 8, Sialoadhesin family member 2, Siglec, SIGLEC8, Siglec-8, SIGLEC8L
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 27181, 292846
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot
• Concentration: 2.23 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide, pH 7.3
• Gene Accession No.: Q9NYZ4
• Gene Symbols: SIGLEC8
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 17-363 of human Siglec-8 (NP_0552572)
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG