Sino Biological™ BACE1 Recombinant Mouse Protein, His Tag Non-distribution product as customer accommodation.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Thr 457) of the mouse BACE1 precursor (NM_011792.5) fused to the polyhistidine tag at the C-terminus

Manufacturer: Invitrogen 50002M08H5

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Catalog No. 50-002-M08H5

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For Research Use Only. Not for use in diagnostic procedures.


    For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
    Format Lyophilized
    Molecular Weight 49.8kDa
    Purity ≥90% by SDS-PAGE
    Quantity 5μg
    Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
    Protein Subtype Proteases
    Endotoxin Concentration <1 EU/μg
    Gene Alias Bace1, C76936
    Common Name beta-site APP cleaving enzyme 1
    Gene Symbol BACE1
    Protein Tag His Tag
    Validated Application Assay Standard
    Protein Family Other Proteins
    Expression System Mammalian

    Beta-site APP-cleaving enzyme 1 (BACE1), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

    • Activity: Measured by its ability to cleave a fluorescent peptide substrate Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dpn)-Arg-Arg-NH2, cleavage of this substrate can be measured using excitation and emission wavelengths of 320 and 405nM, respectively; the specific activity is >2pmole/min./µg
    • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
    • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

    This protein is manufactured by Sino Biological Inc.

  • Description & Specifications

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