Sino Biological CNTF Recombinant Human Protein, N-His Tag
This recombinant protein was expressed from a DNA sequence encoding the human CNTF (NP_000605.1) (Ala 2-Met 200) fused to a polyhistide tag at the N-terminus
Manufacturer: Invitrogen 11841H07E50
DescriptionCiliary Neurotrophic Factor (CNTF), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.
- Activity: Measured by its binding ability in a functional ELISA: 1. Immobilized human CTNFR at 10µg/mL (100µL/well) can bind human CNTF with a linear ranger of 1.28-160ng/mL; 2. Immobilized Rat CTNFR at 10µg/mL (100µL/well) can bind human CNTF with a linear range of 1.28-160ng/mL
- Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
- Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
|Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories|
|Ciliary neurotrophic factor|
|≥90% by SDS-PAGE|
|Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.|
|Cell Structure/Organelle Proteins|
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