Sino Biological CPA2 Recombinant Human Protein, His Tag
This recombinant protein was expressed from a DNA sequence encoding the pre pro form of human CPA2 (NP_001859.1) (Met 1-Tyr 417) fused to a polyhistidine tag at the C-terminus
Manufacturer: Invitrogen 10499H08H50
DescriptionCarboxypeptidase A2 (pancreatic) (CPA2), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.
- Activity: Measured by its ability to cleave a colorimetric peptide substrate, N-acetyl-Phe-Thiaphe-OH (N-Ac-PSP, Peptide International Cat. No. STP-3621-PI), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB), as measured using the wavelength at 405nM and the extinction coefficient of 13,260 M-1 cm-1, the specific activity is >4nM/min./µg
- Formulation: Lyophilized in 25mM Tris, 0.15M NaCl, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
- Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
|Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories|
|≥85% by SDS-PAGE|
|Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.|
|Carboxypeptidase A2 ( CPA2 ) Protein|
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