Sino Biological DKK1 Recombinant Human Protein, His Tag
This recombinant protein was expressed from a DNA sequence encoding the human DKK1 precursor (NP_036374.1) (Met 2-His 266) fused to a polyhistidine tag at the C-terminus
Manufacturer: Invitrogen 10170H08H50
DescriptionDickkopf 1 homolog (Xenopus laevis) (DKK1), recombinant rhesus protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.
- Activity: Measured by its ability to inhibit WNT3A-induced alkaline phosphatase production by C3H10T1/2 cells; the ED50 for this effect is approximately 0.1-0.4µg/mL in the presence of 100ng/mL of mouse Wnt3a
- Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
- Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
|Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories|
|≥85% by SDS-PAGE|
|Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.|
|Dickkopf 1 homolog (Xenopus laevis)|
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