EGF Recombinant Human Protein, Invitrogen Sino Biological
This recombinant protein was expressed from a DNA sequence encoding the mature form of human EGF (NP_001954.2) (Asn 971-Arg 1023) retaining the initial Met at the N-terminus
Manufacturer: Invitrogen 10605HNAE50
DescriptionEpidermal growth factor (EGF) recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.Background: Human epidermal growth factor (EGF) exerts actions on a wide variety of biological processes, and plays an important role in the regulation of cell growth, proliferation, and differentiation mediated by its high-affinity receptor EGFR on the cell surface. It induces differentiation of specific cells, promotes angiogenesis, and inhibits gastric acid secretion in vivo, while serving as a potent mitogenic actor for a variety of cultured cells of both ectodermal and mesodermal origin. Human EGF was originally isolated from urine based on its inhibitory effect on gastric secretion and named urogastrone accordingly. EGF is the founding member of the EGF-family of proteins which are characterized by the presence of at least one EGF structural unit (a conserved six cysteine motif that forms three disulfide bonds). The EGF precursor is believed to exist as a membrane-bound molecule containing nine EGF units, and is proteolytically cleaved to generate the 53-amino acid peptide hormone.
- N-terminal Sequence Analysis: Met 1
- Activity: Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts (Rubin, J.S. et al. 1991, Proc. Natl. Acad. Sci. USA 88:415.), the EC50 for this effect is typically 20-100pg/mL
- Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% mannitol, 5% trehalose, 0.02% Tween-80
- Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
Manufactured by Sino Biological (China).
|Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories|
|EGF, HOMG4, URG|
|Growth Factors & Receptors|
|≥90% by SDS-PAGE|
|Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.|
|EGF (Epidermal Growth Factor)|
|Epidermal growth factor|
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For Research Use Only. Not for use in diagnostic procedures.