Sino Biological™ FCGR2A (CD32A) Recombinant Human Protein, His.AVI Tag, Biotinylated Non-distribution product as customer accommodation.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Met 210) of human FCGR2A (AAA35827.1) (167Arg/His) fused to a C-terminal polyhistidine tagged AVI tag at the C-terminus

Manufacturer: Invitrogen 10374H27H1B25

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Catalog No. 10-374-H27H1L

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For Research Use Only. Not for use in diagnostic procedures.


    For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
    Format Lyophilized
    Molecular Weight 23.6kDa
    Purity ≥90% by SDS-PAGE
    Quantity 5 x 5μg
    Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
    Protein Subtype Fc Receptors
    Endotoxin Concentration <1 EU/μg
    Gene Alias RP11-5K23.6, CD32, CD32A, CDw32, FCG2, FCGR2, FCGR2A, FCGR2A1, FcGR, IGFR2, mgC23887
    Common Name Fc gamma receptor II-a
    Gene Symbol FCGR2A
    Protein Tag His Tag/AVI Tag
    Validated Application Assay Standard
    Protein Family CD Proteins
    Expression System Mammalian

    Fc Gamma Receptor II-A (FCGR2A), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications.

    • The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme)
    • Activity: 1.Measured by its binding ability in a functional ELISA; immobilized human IgG2 at 10µg/mL (100µL/well) can bind biotinylated human FCGR2A with a linear range of 0.16-0.8µg/mL
    • 2. Using the Octet RED System, the recombinant human FCGR2A and BirA bound to human IgG; the affinity constant (Kd) was 12nM
    • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
    • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

    This protein is manufactured by Sino Biological Inc.

  • Description & Specifications

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