Sino Biological™ FLT1 (VEGFR1) Recombinant Human Protein (without Catalytic Activity Domain), His Tag Non-distribution product as customer accommodation.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Asn 756) of human FLT1 (P17948-1) fused to a polyhistidine tag at the C-terminus

Manufacturer: Invitrogen 10136H08H250

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Catalog No. 10-136-H08HL

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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


    Specifications

    For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
    Format Lyophilized
    Gene ID (Entrez) 2321
    Molecular Weight 83.7kDa
    Purity ≥85% by SDS-PAGE
    Quantity 5 x 50μg
    Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
    Structural Form Truncated/Recombinant
    Protein Subtype Receptor Tyrosine Kinases|Growth Factor Receptors
    Endotoxin Concentration <1 EU/μg
    Gene Alias FLT, VEGFR1, FLT1
    Common Name fms-related tyrosine kinase 1
    Gene Symbol FLT1
    Protein Tag His Tag
    Validated Application Assay Standard
    Protein Family Growth Factors & Receptors
    Expression System Mammalian
    KinaseFamily VEGF Receptors

    Fms-related tyrosine kinase 1 (FLT1/VEGFR1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. Since this product is a truncated protein and does not contain intracellular catalytic activity domain, it is not suitable for use in enzyme activity studies.

    • Activity: Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC) (Conn, G. et al., 1990 , Proc. Natl. Acad. Sci. USA 87:1323); the ED50 for this effect is typically 5-30ng/mL in the presence of 5ng/mL human VEGF165
    • Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween-80
    • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

    Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.

  • Description & Specifications

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