Sino Biological HTRA2 Recombinant Human Protein, His Tag
This recombinant protein was expressed from a DNA sequence encoding the mature form of human HTRA2 (O43464-1) (Ala 134-Glu 458) fused to a polyhistide tag at the C-terminus
Manufacturer: Invitrogen 10619H08E25
DescriptionHTRA Serine Peptidase 2 (HTRA2), recombinant human protein is supplied in frozen format. It is suitable for use in protein studies such as enzymatic analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.
- Activity: Protease activity demonstrated by HTRA2 cleavage of bovine beta-casein (Sigma Cat. No. C-6905); incubation of beta-casein at 0.2µg/mL with Recombinant Human HTRA2 at 0.02µg/mL (ratio of 10:1) for 60 minutes at 45°C in 50mM Tris, pH 8.0, which results in >95% cleavage of beta-casein, as revealed by SDS-PAGE
- Formulation: 50mM Tris, 0.3M NaCl, 1mM DTT, 0.2µg/µL BSA, 20% glycerol, pH 7.8
- Reconstitution: No reconstitution needed
|Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories|
|5 x 5μg|
|HtrA serine peptidase 2|
|≥85% by SDS-PAGE|
|Store at -80°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.|
|OMI, PARK13, PRSS25|
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