Sino Biological IGFBP3 Recombinant Human Protein, His Tag
This recombinant protein was expressed from a DNA sequence encoding the mature form of human IGFBP3 (NP_000589.2), residues 28 to 291, fused to a polyhistidine tag at the N-terminus
Manufacturer: Invitrogen 10430H07H50
DescriptionInsulin-like growth factor binding protein 3 (IGFBP3), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications.
- Activity: Measured by its binding ability in a functional ELISA: 1. Immobilized human IGF2 at 10µg/mL (100µL/well) can biotinylated human IGFBP3-His, the ED50 of can biotinylated human IGFBP3-His is 18ng/mL; 2. Immobilized human IGF1 at 10µg/mL (100µL/well) can biotinylated human IGFBP3-His, the ED50 of can biotinylated human IGFBP3-His is 24ng/mL
- Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
- Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
|Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories|
|≥85% by SDS-PAGE|
|Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.|
|IGFs (Insulin-Like Growth Factors)|
|IGFBP3, tcag7.703, BP-53, IBP3|
|Growth Factors & Receptors|
|Insulin-like growth factor binding protein 3|
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