Sino Biological™ IL1A Recombinant Mouse Protein Non-distribution product as customer accommodation.

This recombinant protein was expressed from a DNA sequence encoding the mature form of mouse IL1A (NP_034684.2) (Ser 115-Ser 270), with an initial Met at the N-terminus

Manufacturer: Invitrogen 50114MNAE25

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Catalog No. 50-114-MNAE25

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For Research Use Only. Not for use in diagnostic procedures.

Includes: Five vials of lyophilized protein


    For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
    Format Lyophilized
    Molecular Weight 18kDa
    Purity ≥90% by SDS-PAGE
    Quantity 5 x 5μg
    Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
    Protein Subtype Interleukins
    Gene Alias RP23-160G19.8, Il-1a
    Common Name Interleukin 1 alpha
    Gene Symbol IL1A
    Protein Tag Untagged
    Validated Application Assay Standard
    Protein Family Cytokines & Receptors
    Expression System E. coli

    Interleukin 1 alpha (IL1A), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.

    • Activity: 1. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells, the ED50 for this effect is typically 2-10pg/mL; 2. Measured by its binding ability in a functional ELISA; immobilized mouse IL1A at 10µg/mL (100µL/well) can bind mouse IL1R1, the EC50 of mouse IL1R1 is 0.13µg/mL
    • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
    • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

    This protein is manufactured by Sino Biological Inc.

  • Description & Specifications

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