Sino Biological™ IL1B Recombinant Human Protein Non-distribution product as customer accommodation.

This recombinant protein was expressed from a DNA sequence encoding the mature form of human IL1B (NP_000567.1) (Ala 117-Ser 269)

Manufacturer: Invitrogen 10139HNAE50

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Catalog No. 10-139-HNAE50

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For Research Use Only. Not for use in diagnostic procedures.


    For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
    Format Lyophilized
    Gene ID (Entrez) 3553
    Molecular Weight 17kDa
    Purity ≥90% by SDS-PAGE
    Quantity 50μg
    Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
    Gene Alias IL-1, IL1-BETA, IL1F2, IL1B
    Common Name Interleukin 1, beta
    Gene Symbol IL1B
    Protein Tag Untagged
    Validated Application Assay Standard
    Protein Family Cytokines & Receptors
    Expression System E. coli

    Interleukin 1, beta (IL1B), a recombinant human protein, is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as immunogens, protein standards, and in other cell biology research applications.

    • Activity: 1.Measured by its binding ability in a functional ELISA; a. Immobilized recombinant human IL1B at 2µg/mL (100µL/well) can bind recombinant human IL1R2 with a linear range of 6.25-400ng/mL; b. Immobilized human IL1B at 10µg/mL (100µL/well) can bind human IL1R1, the EC50 of human IL1R1 is 0.48µg/mL; 2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells, the ED50 for this effect is typically 0.5-5pg/mL
    • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
    • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

    This protein is manufactured by Sino Biological Inc.

  • Description & Specifications

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