Sino Biological IL1B Recombinant Human Protein
This recombinant protein was expressed from a DNA sequence encoding the mature form of human IL1B (NP_000567.1) (Ala 117-Ser 269)
Manufacturer: Invitrogen 10139HNAE250
DescriptionInterleukin 1, beta (IL1B), a recombinant human protein, is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as immunogens, protein standards, and in other cell biology research applications.
- Activity: 1.Measured by its binding ability in a functional ELISA; a. Immobilized recombinant human IL1B at 2µg/mL (100µL/well) can bind recombinant human IL1R2 with a linear range of 6.25-400ng/mL; b. Immobilized human IL1B at 10µg/mL (100µL/well) can bind human IL1R1, the EC50 of human IL1R1 is 0.48µg/mL; 2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells, the ED50 for this effect is typically 0.5-5pg/mL
- Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
- Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
|Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories|
|≥90% by SDS-PAGE|
|Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.|
|Interleukin 1, beta|
|5 x 50μg|
|IL-1, IL1-BETA, IL1F2, IL1B|
|Cytokines & Receptors|
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