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Sino Biological™ IL6 Recombinant Human Protein Non-distribution product as customer accommodation.

This recombinant protein is expressed from a DNA sequence encoding the mature form of human IL6 (NP_000591.1) (Val 30-Met 212) retaining the initial Met at the N-terminus

Manufacturer:  Invitrogen 10395HNAE25

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Catalog No. 10-395-HNAE25


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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Specifications

Description

Interleukin 6 (IL-6) recombinant human protein is supplied as a lyophilized powder. This protein is suitable for use in analysis of protein structure and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.Background: Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, and is known particularly for its role in the immune response and acute phase reactions. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity. IL-6 is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule with the molecular mass of 22-28kDa. Three IL-6 signaling pathways (STAT1, STAT3, and Ras-dependent MAPK cascade) have been reported. Dysregulated production of IL-6 and IL6R are implicated in the pathogenesis of several inflammatory diseases and malignancies such as multiple myeloma, rheumatoid arthritis, or osteoporosis.
  • N-terminal Sequence Analysis: Met
  • Activity: 1. Measured by its binding ability in a functional ELISA; immobilized recombinant human IL6 (100µL/well of a 8µg/mL solution) has been shown to bind recombinant human IL6R with a linear range of 1.25-20.0ng/mL; 2. Measured in a cell proliferation assay using TF1 human erythroleukemic cells, the EC50 for this effect is typically 0.2-0.8ng/mL
  • Formulation: lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% mannitol, 5% trehalose, 0.02% Tween®-80
  • Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
Description & Specifications

Specifications

Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
20.3kDa
5 x 5μg
Full Length/Recombinant
BSF2, HGF, HSF, IFNB2, IL-6
IL6
Cytokines & Receptors
Untagged
Lyophilized
≥95% by SDS-PAGE
Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Interleukins
Interleukin 6 (interferon, beta 2)
Assay Standard
E. coli
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