Sino Biological IL6ST Recombinant Mouse Protein, His Tag
This recombinant protein was expressed from a DNA sequence encoding the mouse extracellular domain (Met 1-Glu 617) of IL6ST (NP_034690.3) fused to a polyhistidine tag at the C-terminus
Manufacturer: Invitrogen 50135M08H250
DescriptionInterleukin 6 signal transducer (IL6ST), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.
- Activity: 1. Measured by its ability to bind mouse IL11RA in a functional ELISA; 2. Measured by its ability to inhibit the IL6R enhancement of IL6 activity on M1 mouse myeloid leukemia cells (Saito, T. et al. 1991, J. Immunol. 147:168), the ED50 for this effect is typically 0.2-0.8µg/mL in the presence of 50ng/mL recombinant human IL6sR and 100ng/mL recombinant human IL6
- Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
- Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
|Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories|
|≥90% by SDS-PAGE|
|Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.|
|IL6ST, 5133400A03Rik, AA389424, BB405851, CD130, D13Ertd699e, gp130|
|Cytokines & Receptors/CD Proteins|
|5 x 50μg|
|Interleukin 6 signal transducer|
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