Sino Biological™ KIT (c-Kit) Recombinant Human Protein (without Catalytic Activity Domain), His Tag Non-distribution product as customer accommodation.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human KIT isoform 2 (P10721-2) (Met 1-Thr 516) fused to a polyhistidine tag at the C-terminus

Manufacturer: Invitrogen 50530M02H5

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Catalog No. 50-530-M02H5

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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


    Specifications

    For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
    Format Lyophilized
    Gene ID (Entrez) 16590
    Molecular Weight 82.5kDa
    Purity ≥85% by SDS-PAGE
    Quantity 5μg
    Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
    Structural Form Truncated/Recombinant
    Protein Subtype Receptor Tyrosine Kinases|Cytokine Receptors
    Endotoxin Concentration <1 EU/μg
    Gene Alias Bs, CD117, Fdc, Gsfsco1, Gsfsco5, Gsfsow3, SCO1, SCO5, SOW3, Ssm, Tr-kit, W, c-KIT
    Common Name KIT oncogene
    Gene Symbol KIT
    Protein Tag IgG1 Fc Tag
    Validated Application Assay Standard
    Protein Family Growth Factors & Receptors/CD Proteins
    Expression System Mammalian
    KinaseFamily PDGFR Kinase Family

    Kit oncogene (KIT/c-Kit), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. Since this product is a truncated protein and does not contain intracellular catalytic activity domain, it is not suitable for use in enzyme activity studies.

    • Activity: Measured by its binding ability in a functional ELISA; immobilized human KIT at 20µg/mL (100µL/well) can bind biotinylated mouse KITL with a linear range of 1.28-6.4ng/mL
    • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween-80
    • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

    Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.

  • Description & Specifications

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