Sino Biological KITL Recombinant Mouse Protein, His Tag
This recombinant protein was expressed from a DNA sequence encoding the mouse KITL (P20826-1) (Met 1-Ala 189) fused to a polyhistidine tag at the C-terminus
Manufacturer: Invitrogen 50487M08B25
DescriptionKIT ligand (KITL), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.
- Activity: 1. Measured by its binding ability in a functional ELISA; immobilized mouse KITL at 2µg/mL (100µL/well) can bind mouse KIT with a linear ranger of 1.28-32ng/mL; 2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells (Kitamura, T. et al. 1989, J. Cell Physiol. 140:323), the EC50 for this effect is typically 0.5-5ng/mL
- Formulation: Lyophilized in 20mM Tris, 500mM NaCl, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
- Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
|Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories|
|5 x 5μg|
|Truncated/Recombinant/Cell Surface Protein|
|≥90% by SDS-PAGE|
|Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.|
|Other Growth Factors|
|Clo, Con, Gb, Kitlg, mgf, SCF, SF, SLF, Sl, Steel, c ontrasted|
|Growth Factors & Receptors|
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