Sino Biological™ KITL Recombinant Mouse Protein, His Tag Non-distribution product as customer accommodation.

This recombinant protein was expressed from a DNA sequence encoding the mouse KITL (P20826-1) (Met 1-Ala 189) fused to a polyhistidine tag at the C-terminus

Manufacturer: Invitrogen 50487M08B50

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Catalog No. 50-487-M08B50

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For Research Use Only. Not for use in diagnostic procedures.

Includes: One vial of lyophilized protein


    For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
    Format Lyophilized
    Molecular Weight 19.7kDa
    Purity ≥90% by SDS-PAGE
    Quantity 50μg
    Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
    Structural Form Truncated/Recombinant/Cell Surface Protein
    Protein Subtype Other Growth Factors
    Endotoxin Concentration <1 EU/μg
    Gene Alias Clo, Con, Gb, Kitlg, mgf, SCF, SF, SLF, Sl, Steel, c ontrasted
    Common Name Kit ligand
    Gene Symbol KITL
    Protein Tag His Tag
    Validated Application Assay Standard
    Protein Family Growth Factors & Receptors
    Expression System Insect

    KIT ligand (KITL), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.

    • Activity: 1. Measured by its binding ability in a functional ELISA; immobilized mouse KITL at 2µg/mL (100µL/well) can bind mouse KIT with a linear ranger of 1.28-32ng/mL; 2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells (Kitamura, T. et al. 1989, J. Cell Physiol. 140:323), the EC50 for this effect is typically 0.5-5ng/mL
    • Formulation: Lyophilized in 20mM Tris, 500mM NaCl, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
    • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

    This protein is manufactured by Sino Biological Inc.

  • Description & Specifications

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