Sino Biological LEP (Leptin) Recombinant Human Protein
This recombinant protein was expressed from a DNA sequence encoding the mature form of human Leptin (NP_000221.1), residues 22 to 167, containing an additional Met
Manufacturer: Invitrogen 10221HNAE250
DescriptionLeptin (LEP), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications.
- Activity: Measured by its binding ability in a functional ELISA: 1. Immobilized human Leptin at 1.25µg/mL (100µL/well) can bind human Leptin receptor-Fc with a linear range of 0.032-4.0µg/mL; 2. Immobilized human Leptin at 5µg/mL (100µL/well) can bind human Leptin receptor-His with a linear range of 0.032-4.0µg/mL
- Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
- Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
|Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories|
|≥95% by SDS-PAGE|
|Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.|
|Lectins & Leptins|
|5 x 50μg|
|FLJ94114, OB, OBS|
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