Sino Biological MAP1D Recombinant Human Protein, His Tag
This recombinant protein was expressed from a DNA sequence encoding the human METAP1D (NP_954697.1) (Arg 44-Ala 335) with a polyhistidine tag at the C-terminus and an initial Met an the N-terminus
Manufacturer: Invitrogen 10883H08B250
DescriptionMethionyl aminopeptidase type 1D (mitochondrial) (METAP1D), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.
- Activity: Measure by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly-Pro-AMC; the resulting Gly-Pro-AMC is cleaved by human DPP4/CD26, the specific activity is >30pmole/min./µg
- Formulation: Lyophilized in 50mM Tris, 100mM NaCl, pH 8.0, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
- Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
|Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories|
|5 x 50μg|
|≥90% by SDS-PAGE|
|Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.|
|Methionyl aminopeptidase type 1D (mitochondrial)|
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