Sino Biological™ MCAM (CD146) Recombinant Human Protein, hIgG1-Fc Tag Non-distribution product as customer accommodation.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gly 559) of human MCAM precursor (NP_006491.2) fused to the Fc region of human IgG1 at the C-terminus

Manufacturer: Invitrogen 10115H02H5

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Catalog No. 10-115-H02H5

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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


    Specifications

    For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
    Format Lyophilized
    Gene ID (Entrez) 4162
    Molecular Weight 86.4kDa
    Purity ≥90% by SDS-PAGE
    Quantity 5μg
    Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
    Structural Form Truncated/Recombinant
    Protein Subtype Other Proteins
    Endotoxin Concentration <1 EU/μg
    Gene Alias MCAM, CD146, MUC18
    Common Name Melanoma cell adhesion molecule
    Gene Symbol MCAM
    Protein Tag IgG1 Fc Tag
    Validated Application Assay Standard
    Protein Family CD Proteins
    Expression System Mammalian

    Melanoma cell adhesion molecule (MCAM/CD146), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in cell biology research applications. It can also be used as a protein standard.

    • Activity: Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line; when 3 x 104 cells/well are added to human CD146 coated plates (0.8µg/mL, 100µL/well), approximately >30 % will adhere after one hour at 37°C
    • Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
    • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

    Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.

  • Description & Specifications

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