Sino Biological MCAM Recombinant Human Protein, His Tag
This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human MCAM precursor (NP_006491.2) (Met 1-Gly 559) fused to a C-terminal polyhistidine tag
Manufacturer: Invitrogen 10115H08H5
DescriptionMelanoma Cell Adhesion Molecule (MCAM), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.
- Activity: Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line; when 3 x 104 cells/well are added to human MCAM coated plates (0.8µg/mL, 100µL/well), approximately >50% will adhere after one hour at 37°C
- Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
- Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
|Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories|
|Intercellular Adhesion Molecule|
|MCAM, CD146, MUC18|
|≥90% by SDS-PAGE|
|Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.Expires one year from date of receipt when stored as instructed.|
|Melanoma Cell Adhesion Molecule|
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