Sino Biological™ NGF Recombinant Human Protein Non-distribution product as customer accommodation.

This recombinant protein was expressed from a DNA sequence encoding the mature form of human NGF (NP_002497.2) (Ser 122-Arg 239)

Manufacturer: Invitrogen 11050HNAC250

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Catalog No. 11-050-HNACL

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For Research Use Only. Not for use in diagnostic procedures.


    Specifications

    For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
    Format Lyophilized
    Molecular Weight 13.2kDa
    Purity ≥95% by SDS-PAGE
    Quantity 5 x 50μg
    Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
    Structural Form Full Length/Recombinant
    Protein Subtype NGFs (Nerve Growth Factors)
    Endotoxin Concentration <1 EU/μg
    Gene Alias NGF, Beta-NGF, HSAN5, mgC161426, mgC161428, NGFB
    Common Name Nerve growth factor (beta polypeptide)
    Gene Symbol NGF
    Protein Tag Untagged
    Validated Application Assay Standard
    Protein Family Growth Factors & Receptors
    Expression System Mammalian

    Nerve Growth Factor (NGF) recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.Background: As the first one of a series of neurotrophic factors, nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF was identified as a large complex consisting of three noncovalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF, was demonstrated to exhibit the growth stimulating activity of NGF. β-NGF is a target-derived secreted homodimeric protein of two 120-amino acid mature polypeptides after proteolytic removal of the signal and pro peptide. NGF acts via at least two receptors on the surface of cells, TrkA and p75 receptors to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. In additon to its neurotropic activities, studies indicated that NGF may also have an important role in the regulation of the immune system. The synthesis of β-NGF in astrocytes is enhanced by various other cytokines including IL-1, TNF-α, PDGF and TGF-β. Defects in β-NGF are the cause of hereditary sensory and autonomic neuropathy type 5 (HSAN5) characterized by degeneration of dorsal root and autonomic ganglion cells, as well as sensory and/or autonomic abnormalities.

    • N-terminal Sequence Analysis: Ser 122
    • Activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells; the EC50 for this effect is typically 0.4-1.0ng/mL
    • Formulation: Lyophilized in 50mM NaAc, 150mM NaCl, pH 5.5, 5% mannitol, 5% trehalose, 0.02% Tween®-80
    • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

    Manufactured by Sino Biological (China).

  • Description & Specifications

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