Sino Biological NGF Recombinant Mouse Protein
This recombinant protein was expressed from a DNA sequence encoding the mature form of mouse NGF (NP_001106168.1), residues 122 to 241, with an initial Met at the N-terminus
Manufacturer: Invitrogen 50385MNAC5
DescriptionNerve growth factor (NGF), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in performing cell based assays. It can also be used as a protein standard, or in other research applications.
- Activity: Measured in a cell proliferation assay using TF1 human erythroleukemic cells; the ED50 for this effect is 0.2-2.0ng/mL
- Formulation: Lyophilized in 50mM Na acetate buffer, pH 5.5, 150mM NaCl, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
- Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
|Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories|
|≥95% by SDS-PAGE|
|Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.|
|Growth Factors & Receptors|
|Nerve growth factor|
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