Sino Biological PECAM1 Recombinant Human Protein, hIgG1-Fc Tag
This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Lys 601) of human PECAM1 (EAW94208.1) fused to the fused Fc region of human IgG1 at the C-terminus
Manufacturer: Invitrogen 10148H02H5
DescriptionPlatelet/Endothelial Cell Adhesion Molecule 1 (PECAM1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.
- Activity: Measured by its ability of the immobilized protein to support the adhesion of Jurkat human leukemic T cells; when cells are added to recombinant human PECAM1 coated plates (3.3µg/mL, 100µL/well) in the presence of PMA, approximately 50%-65% will adhere specifically after 30 minutes at 37°C
- Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
- Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
|Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories|
|Intercellular Adhesion Molecule|
|PECAM1, CD31, PECAM-1|
|IgG1 Fc Tag|
|≥90% by SDS-PAGE|
|Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.|
|Platelet/endothelial cell adhesion molecule 1|
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