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Sino Biological™ SELE (E-SELECTIN) Recombinant Rat Protein, His Tag Non-distribution product as customer accommodation.

This recombinant protein was expressed from a DNA sequence encoding the rat SELE (P98105-1) extracellular domain (Met 1-Pro 494) fused to a polyhistidine tag at the C-terminus

Manufacturer:  Invitrogen 80033R08H25

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Catalog No. 80-033-R08H25


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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Includes: Five vials of lyophilized protein

Specifications

Description

Selectin E (SELE), recombinant rat protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.
  • Activity: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells; when 5 x 104 cells/well are added to rat SELE coated plates (2µg/mL with 100µL/well), approximately 20-35% will adhere for 1 hour incubation at 37°C
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
Description & Specifications

Specifications

Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
53kDa
5 x 5μg
Other Proteins
SELE
SELE
Other Proteins
His Tag
Lyophilized
≥90% by SDS-PAGE
Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
<1 EU/μg
Sele–selectin E
Assay Standard
Mammalian
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