Sino Biological™ SERPINA1 Recombinant Human Protein, His Tag Non-distribution product as customer accommodation.

This recombinant protein was expressed from a DNA sequence encoding the human SERPINA1 pre-protein (NP_000286.3) (Met 1-Lys 418) fused to the polyhistidine tag at the C-terminus

Manufacturer: Invitrogen 10306H08H25

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Catalog No. 10-306-H08H25

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For Research Use Only. Not for use in diagnostic procedures.


    For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
    Format Lyophilized
    Molecular Weight 45.7kDa
    Purity ≥90% by SDS-PAGE
    Quantity 5 x 5μg
    Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
    Protein Subtype Proteinase Inhibitor
    Endotoxin Concentration <1 EU/μg
    Gene Alias SerpinA1, PI, A1A, AAT, PI1, A1AT, mgC9222, PRO2275, mgC23330
    Common Name Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
    Gene Symbol SERPINA1
    Protein Tag His Tag
    Validated Application Assay Standard
    Protein Family Protease Inhibitors
    Expression System Mammalian

    Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

    • Activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2
      ; the IC50 value is <3.0nM, as measured in 100µL reaction mixture containing 1.25ng trypsin, 10µM substrate, 50mM Tris, 10mM CaCl2, 0.15 M NaCl, pH 7.5
    • Formulation: Lyophilized in 25mM Tris, 150mM NaCl, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
    • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

    This protein is manufactured by Sino Biological Inc.

  • Description & Specifications

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