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Sino Biological™ TFPI Recombinant Human Protein, His Tag Non-distribution product as customer accommodation.

This recombinant protein was expressed from a DNA sequence encoding the human TFPI (NP_006278.1) (Met 1-Lys 282) fused to a C-terminal polyhistidine tag

Manufacturer:  Invitrogen 10564H08H50

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Catalog No. 10-564-H08H50


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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Specifications

Description

Tissue Factor Pathway Inhibitor (Liporeotein-Associated Coagulation Inhibitor) (TFPI), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as enzymatic analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.
  • Activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec Cat. No.27114), the IC50 value is <0.4nM, as measured in 100µL reaction mixture containing 1.25ng trypsin (Sigma Cat. No.T4799), 10µM substrate, 50mM Tris, 10mM CaCl2, 0.15M NaCl, 0.05% Brij-35, pH 7.5
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
Description & Specifications

Specifications

Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
30.6kDa
50μg
Proteinase Inhibitor
EPI, LACI, TFI, TFPI1
TFPI
Protease Inhibitors
His Tag
Lyophilized
>85% by SDS-PAGE
Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
<1 EU/μg
Tissue factor pathway inhibitor (liporeotein-associated coagulation inhibitor)
Assay Standard
Mammalian
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