Sino Biological TNF (TNFA) Recombinant Canine Protein
This recombinant protein was expressed from a DNA sequence encoding the soluble form of canine TNFα (NP_001003244.4) (Val 77-Leu 233) fused to an initial Met at the N-terminus
Manufacturer: Invitrogen 70003DNAE25
DescriptionTumor Necrosis Factor (TNF), recombinant canine protein is supplied in frozen format. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.
- Activity: Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D; the ED50 is typically 0.2-1.2ng/mL
- Formulation: 50mM Tris, 500mM NaCl, 20% glycerol, pH 8.0
- Reconstitution: No reconstitution needed
|Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories|
|5 x 5μg|
|TNF (Tumor Necrosis Factor)|
|Tumor necrosis factor|
|≥90% by SDS-PAGE|
|Store at -80°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.|
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